How does PCR detect the virus?

How does PCR detect the virus?

To detect this type of virus in the body using RT-PCR in real time, scientists must convert its RNA into DNA in a process called “reverse transcription”. This step is necessary because only DNA can be copied, or “amplified”, in the laboratory.

What are the steps of PCR?

Each round of PCR is made up of three steps : denaturation of the DNA by heating to separate the two strands which compose it, hybridization of the primers at the ends of the sequence sought, then elongation thanks to the action of a DNA polymerase*.

How does the antigen test work?

As the test RT-PCR, it consists of a nasal sample with a swab. With a result available in 15 to 30 minutes, these testing allow the immediate implementation of isolation and contact tracing measures.

Why is the PCR test more reliable?

the PCR test searches for the RNA of the virus then the test antigen searches for its proteins. They are used to know if the person is currently infected with the Sars-Cov-2 virus. the PCR test has an efficiency of 95% while the test antigenic is 70-80% effective and especially for symptomatic cases.

How do I know if I have been infected?

These may include: cough, sore throat, difficulty breathing, fever or feeling feverish, muscle pain, sudden loss of smell and/or taste. More rarely: headaches, digestive symptoms, conjunctivitis, colds. In the elderly, confusion can also be observed.

Why use PCR for bacteria research?

WHY “WANT” TO USE PCR FOR THE RESEARCH OF BACTERIA? PCR has a number of advantages…and a number of disadvantages over conventional (and gold standard) techniques used in the research and identification of bacteria.

What is PCR?

Comparison (very theoretical) of traditional methods for identifying bacteria with a PCR approach. Traditional techniques for identifying bacteria are still used, most of the time, as reference techniques. In some cases they are advantageously replaced and/or supplemented by PCR.

How to identify a bacterium?

To identify a bacterium, you need to perform staining tests, analyze its appearance and observe how well it reacts to different conditions. If you need a quick result, take a DNA sample to send to a lab.

What are the benefits of PCR?

PCR can be a very advantageous tool in the detection of mutated genes responsible for cancers and genetic diseases. It can also be very useful for monitoring cancer therapies and thus allow the doctor to continue treatment or not.