What are the stages of chromatography?

What are the stages of chromatography?

What are the stages of chromatography?

The CCM takes place in three steps : cuvette preparation, plate preparation, and elution.

What is Retention Factor?

The retention factor characterizes the retention of a compound. It is an important parameter independent of the flow (Be careful not to confuse with the distribution coefficient K!) For values ​​of R greater than 1.5, the peaks are correctly separated.

What is the retention factor of a solvent?

Thin layer chromatography: the Rf or retention factor 1 nature (and composition) of the solvent 2 nature of the adsorbent 3 thickness of the absorbent layer 4 amount of sample deposited

What is the retention time of a solute?

This is the time taken by the solute to leave the column, ie the time elapsed between the injection and the maximum of the peak of the eluted compound. This is the main retention quantity. It is denoted t R (in minutes). It varies according to the flow rate, the elution temperature, the composition of the mobile phase and the aging of the column.

What is Retention Magnitude?

Definitions of the different retention quantities. Retention time. This is the time taken by the solute to leave the column, ie the time elapsed between the injection and the maximum of the peak of the eluted compound. This is the main retention quantity. It is noted t R (in minutes)