Why Is it necessary to use an electron microscope rather than an optical microscope in the magnification can go up to a few hundred times?

Why Is it necessary to use an electron microscope rather than an optical microscope in the magnification can go up to a few hundred times?

The resolution and the magnification biggest of the electron microscope are due to do than the de Broglie wavelength of an electron is much smaller than the wavelength of a photon of visible light.

How is immersion oil used in microscopy?

Immersion oil increases the resolving power of the microscope by replacing the air gap between the immersion objective and the cover glass with a highly refractive medium and reducing the refraction of light. How is oil immersion used in microscopy?

What are the benefits of immersion oil?

Immersion oil contributes two properties to the image seen under the microscope: finer resolution and brightness. These properties are most critical at high magnification; Therefore, only short throw and higher power lenses are generally designed for oil immersion.

What are the best substitutes for commercial immersion oil?

Methyl salicylate, also called wintergreen oil, has proven to be an excellent substitute for commercial immersion oil. Are you using an oil immersion coverslip?

How does beam dipping work?

Beam path with immersion medium (yellow) (left half) and without (right half). The rays (black) that come from the object (red) at a certain angle and pass through the glass cover (orange, just like the slide below) can only enter the objective (dark blue) when the use of immersion.